Putative KASP primers for Axiom SNPs
KASP primers were designed using the offline version of PolyMarker (http://polymarker.tgac.ac.uk)
"Ramirez-Gonzalez, R.H., Uauy, C. and Caccamo, M. (2015) PolyMarker: A fast polyploid primer design pipeline. Bioinformatics. In press. doi:10.1093/bioinformatics/btv069" http://dx.doi.org/10.1093/bioinformatics/btv069.
using the methodology described in:
"Ramirez-Gonzalez R.H., Segovia V., Bird N., Fenwick P., Holdgate S., Berry S., Jack P., Caccamo M.and Uauy C. (2014) RNA-Seq bulked segregant analysis enables the identification of high-resolution genetic markers for breeding in hexaploid wheat. Plant Biotechnol. J., doi: 10.1111/pbi.12281”.
The reference used was the IWSCv1, using the soft masked contains in Ensembl (http://plants.ensembl.org/Triticum_aestivum/Info/Index).
PolyMarker was run by Ricardo H. Ramírez-Gonzalez (ricardo.ramirez-gonzalez@tgac.ac.uk).
Any questions or queries relating to the KASP primer design program or the performance of the primers should be directed to Ricardo Ramirez-Gonzalez and Cristobal Uauy (cristobal.uauy@jic.ac.uk).
All primers: all_axiom_markers.csv
Based at the University of Bristol with support from BBSRC.
Maintained by Mark Winfield.