TaqMan® SNPs Summary Statistics
Total number of markers available: 4,800
Mapped markers available: 4,735
TaqMan® Assays are a gold standard in qPCR applications and the technology is based on 5’ nuclease chemistry using specific fluorescent probes to discriminate SNP variants. These assays come as single tube, pre-formulated primer and probe sets which can be used right out of the box requiring no optimization or laborious setup. Assays are available from ThermoFisher Scientific.
TaqMan® Assays were used on a 'core marker set' of 960 SNPs which, based on the Avalon x Cadenza genetic map, were selected to be evenly distributed across the wheat genome.
|A||227 (224)||278 (252)||232 (232)||128 (240)||262 (232)||265 (201)||243 (228)||1635 (1609)|
|B||414 (238)||356 (260)||281 (278)||156 (230)||362 (244)||249 (254)||113 (252)||1931 (1756)|
|D||386 (169)||207 (204)||46 (216)||66 (181)||227 (210)||117 (177)||120 (204)||1169 (1361)|
Table showing the number of TaqMan® SNPs that have been mapped to each of the 21 wheat chromosomes; the expected number, given the relative lengths* of each chromosome and the total number of mapped SNPs (4,735), is shown in parentheses. These data clearly show that far fewer SNPs have been mapped to the D genome than would be expected were the SNPs to be evenly distributed across the genome.
*The total length of the wheat genome (234.4 μm) and the percentage length of each chromosome and genome (A = 34.0% , B = 37.2%, C = 28.8%) were taken from Gill, B.S., Friebe, B. and Endo, T.R. (1991) Standard karyotype and nomenclature system for description of chromosome bands and structural aberrations in wheat, Genome 34: 830-839; the article reports on the relative length in the variety Chinese Spring, which may differ from that for the varieties used in this study.
Maintained by Mark Winfield.