Available TaqMan® assays

Comparisons were performed between genotype calls made using TaqMan® Assay and those obtained using KASP chemistry. Using 20 markers across 10 wheat varieties, concordance was approximately 90% where both platforms were able to successfully make a call (Table below). Interestingly, TaqMan® assays were able to make calls for certain SNP/sample combinations (white cells in the table below, e.g. BS00003717) for which KASP assays were not able to do so. In addition, for non-concordant SNP calls, (pink cells in table, e.g. BS0009377) TaqMan® calls were confirmed using a real-time trace feature in Life Technologies’ ViiA 7™ instrument.

To order these TaqMan® Assays from ThermoFisher Scientific please visit their web site.


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Comparison of TaqMan® and KASP data across 10 wheat varieties. For each variety, the KASP call is shown in the left-hand column and the corresponding TaqMan® call in the right column. Green cells indicate concordant calls, pink cells indicate discordant calls and white cells indicate a result in which only one platform was able to make a genotype call.




Based at the University of Bristol with support from BBSRC. BBSRC icon Bristol icon

Maintained by Mark Winfield.


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