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MuID has been developed for the simultaneous identification of insertion events within large numbers of genes. This allows the characterisation by insertion mutagenesis of genes expressed during specific developmental processes.

Mu flanking sequences are amplified from individual Mu active plants and used as hybridistation probes to detect homologous cDNAs on high density arrays. This method had been applied for the detection of insertions within genes expressed during maize kernel and leaf development, and had aslo identified maize mutator insertion flanking sequences which hybridise with arrayed wheat endosperm cDNAs.

Mu flanking sequences were amplified from an individual, randomly selected maize palnt using MuAFLP. These flanking sequences were radiolabelled and used as a mixed hybridisation probe to identify homologous cDNA sequences within an array of 3072 randomly picked, double spotted maize endosperm cDNA clones. The identified cDNAs were sequenced and used to clone the corresponding mutator insertion event.

Maintained by Gary Barker Last updated Dec 2001